SLAM family member 8 is expressed in and enhances the growth of anaplastic large cell lymphoma
Akihiko Sugimoto, Tatsuki R. Kataoka, Hiroaki Ito, Kyohei Kitamura, Narumi Saito, Masahiro Hirata, Chiyuki Ueshima, Yusuke Takei, Koki Moriyoshi, Yasuyuki Otsuka, Momoko Nishikori, Akifumi Takaori‐Kondo, Hironori Haga
Abstract
Signaling lymphocytic activation molecule family member 8 (SLAMF8) / B-lymphocyte activator macrophage expressed/CD353 is a member of the CD2 family. SLAMF8 suppresses macrophage function but enhances the growth of neoplastic mast cells via SHP-2. In this study, we found that some anaplastic large cell lymphoma (ALCL) samples were immunohistochemically positive for SLAMF8. However, we found no significant differences between SLAMF8-positive and SLAMF8-negative ALCL samples with respect to age, gender, site, or prognosis. We also identified SLAMF8 expression in ALCL cell lines, Karpas299, and SU-DHL-1. SLAMF8 knockdown decreased the activation of SHP-2 and the growth of these cell lines, and increased the apoptosis of these cell lines. In addition, we observed the interaction between SLAMF8 and SHP-2 in these cell lines using the DuoLink in situ kit. Taken together, these results suggest that SLAMF8 may enhance the growth of ALCL via SHP-2 interaction.