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Patient-derived organotypic tumor spheroids, tumoroids, and organoids: advancing immunotherapy using state-of-the-art 3D tumor model systems

David J. Bozym, David X. Zheng, Or‐Yam Revach, Amir R. Aref, Russell W. Jenkins

2025Lab on a Chip10 citationsDOIOpen Access PDF

Abstract

This review provides an overview of state-of-the-art patient-derived 3D tumor models with a focus on patient-derived organotypic tumor spheroids (PDOTS), current preclinical applications, and future directions for preclinical and clinical use.

Topics & Concepts

OrganoidSpheroidImmunotherapyTumor cellsTumor immunologyComputational biologyCancer researchBiologyCell biologyImmunologyImmune systemIn vitroBiochemistryMathematical Biology Tumor GrowthCancer Cells and MetastasisCancer Immunotherapy and Biomarkers