High-Performance Intensiometric Direct- and Inverse-Response Genetically Encoded Biosensors for Citrate
Y Zhao, Yi Shen, Yurong Wen, Robert E. Campbell
Abstract
≈ 9) designated Citron1. We report the X-ray crystal structure of Citron1 and demonstrate the utility of both biosensors for qualitative and quantitative imaging of steady-state and pharmacologically perturbed citrate concentrations in live cells.
Topics & Concepts
BiosensorHistidineMutagenesisChemistryBiochemistryComputational biologyBiologyBiophysicsEnzymeMutationGeneMass Spectrometry Techniques and ApplicationsAdvanced biosensing and bioanalysis techniquesRNA and protein synthesis mechanisms