Activatable endoplasmic reticulum-targeted NIR fluorescent probe with a large Stokes shift for detecting and imaging chymotrypsin
Ting Lan, Qinqin Tian, Minghua Li, Wei He
Abstract
) of 0.9411 and 0.9522 in P815 cells and HepG2 cells, respectively. Moreover, ISO-Chy was successfully utilized to visualize endogenous chymotrypsin in P815 cells and HepG2 cells and was first used to detect chymotrypsin activity in HepG2 tumor-bearing mice. These findings indicate that ISO-Chy could be an effective tool for detecting endogenous chymotrypsin activity, supporting its use for investigating chymotrypsin function in pathologic processes.
Topics & Concepts
ChymotrypsinEndoplasmic reticulumAutofluorescenceFluorescenceFluorophoreChemistryBiophysicsStokes shiftTrypsinBiochemistryEnzymeBiologyOpticsPhysicsMolecular Sensors and Ion DetectionNanoplatforms for cancer theranosticsAdvanced biosensing and bioanalysis techniques