Effects of Waterlogging on Soybean Rhizosphere Bacterial Community Using V4, LoopSeq, and PacBio 16S rRNA Sequence
Taobing Yu, Lang Cheng, Qi Liu, Shasha Wang, Yuan Zhou, Hongbin Zhong, Tang Meifang, Hai Nian, Tengxiang Lian
Abstract
was increased in the rhizosphere in both soil types after suffering waterlogging. Moreover, the core microorganisms obtained by different sequencing methods all contain species related to nitrogen cycling. Together, our study not only explored the role of microorganisms enriched at the rhizosphere level under waterlogging in assisting soybean to resist stress but also showed that LoopSeq sequencing is a less expensive and more convenient method for full-length sequencing by comparing different sequencing methods.
Topics & Concepts
RhizosphereBiologyMicroorganismWaterlogging (archaeology)Microbial population biologyMetagenomics16S ribosomal RNACommunity structureNutrient cycleAgronomyNutrientEcologyBacteriaGeneGeneticsWetlandMicrobial Community Ecology and PhysiologyLegume Nitrogen Fixing SymbiosisGenomics and Phylogenetic Studies