Human PSCs determine the competency of cerebral organoid differentiation via FGF signaling and epigenetic mechanisms
Hirosato Ideno, Kent Imaizumi, Hiroko Shimada, Tsukasa Sanosaka, Akisa Nemoto, Jun Kohyama, Hideyuki Okano
Abstract
locus, and this epigenetic change suppressed the future activation of non-canonical Wnt signaling after differentiation, leading to reliable cerebral organoid generation. This study underscores the importance of PSC culture conditions for directed differentiation into cerebral organoids, and the epigenetic status regulated by FGF signaling is involved in the underlying mechanisms.
Topics & Concepts
OrganoidWnt signaling pathwayFibroblast growth factorEpigeneticsCell biologyInduced pluripotent stem cellBiologyCellular differentiationDNA methylationSignal transductionEmbryonic stem cellGeneticsGene expressionGeneReceptorPluripotent Stem Cells ResearchRenal and related cancersCRISPR and Genetic Engineering