Cyclotriphosphazene-Based “Butterfly” Fluorescence Probe for Lysosome Targeting
Pan Liu, Le Wang, Liang Chen, Xiqi Su, Xiangyang Shi
Abstract
A cyclotriphosphazene-based “butterfly” fluorescence probe HCCP-MNI bearing two naphthalimide and morpholine units were developed for lysosome targeting. The synthesized HCCP-MNI exhibited stable fluorescence signals and was cytocompatible in the given concentration range. Co-localization experimental results showed that cells treated with the HCCP-MNI and a commercial dye (Lyso-Tracker Red DND-99) had overlapped fluorescence signals, demonstrating its targeting specificity to lysosomes. The developed HCCP-MNI may be used for cell tracking applications associated with the functionalities of lysosomes.
Topics & Concepts
ChemistryLysosomeFluorescenceButterflyNanotechnologyBiochemistryOpticsEconomicsFinancePhysicsMaterials scienceEnzymeCalcium signaling and nucleotide metabolismOrganic Light-Emitting Diodes ResearchAdenosine and Purinergic Signaling