Production of L-Theanine Using <i>Escherichia coli</i> Whole-Cell Overexpressing γ-Glutamylmethylamide Synthetase with Baker’s Yeast
Soo-Yeon Yang, Yeong-Hoon Han, Ye‐Lim Park, Jun Young Park, So-young No, Daham Jeong, Saerom Park, Hyung Yeon Park, Wooseong Kim, Seung-Oh Seo, Yung-Hun Yang
Abstract
, respectively. Additionally, ATP was found to be an important factor for producing high concentration of L-theanine so several strains were tested during the reaction for ATP regeneration. Bakers yeast was found to decrease the demand for ATP most effectively. Addition of potassium phosphate source was demonstrated by producing 4-fold higher L-theanine. To enhance the conversion yield, GMAS was additionally overexpressed in the system. A maximum of 198 mM L-theanine was produced with 16.5 mmol/l/h productivity. The whole-cell reaction involving GMAS has greatest potential for scale-up production of L-theanine.