A universal DNA aptamer as an efficient inhibitor against spike-protein/hACE2 interactions
Achut Prasad Silwal, Raunak Jahan, Siddhartha Kalpa Samadhi Thennakoon, Satya Prakash Arya, Rick Mason Postema, Elizabeth Claire Vander Ark, Xiaohong Tan
Abstract
DNA SELEX towards the wild-type (WT) spike-protein. This aptamer, A1C1, binds to the WT spike-protein or other variants of concern such as Delta and Omicron with low nanomolar affinities. A1C1 inhibited the interaction between hACE2 and various spike-proteins by 85-89%. This universal A1C1 aptamer can be used to design diagnostic and therapeutic molecular tools to target SARS-CoV-2 and its variants.
Topics & Concepts
AptamerSpike (software development)DNAChemistryBiophysicsComputational biologyComputer scienceBiologyBiochemistryMolecular biologySoftware engineeringAdvanced biosensing and bioanalysis techniquesDNA and Nucleic Acid ChemistryRNA and protein synthesis mechanisms