Highly Contiguous Nanopore Genome Assembly of Chlamydomonas reinhardtii CC-1690
Samuel O’Donnell, Frédéric Chaux, Gilles Fischer
Abstract
The current Chlamydomonas reinhardtii reference genome remains fragmented due to gaps stemming from large repetitive regions. To overcome the vast majority of these gaps, publicly available Oxford Nanopore Technology data were used to create a new reference-quality de novo genome assembly containing only 21 contigs, 30/34 telomeric ends, and a genome size of 111 Mb.
Topics & Concepts
Chlamydomonas reinhardtiiGenomeContigSequence assemblyNanopore sequencingNanoporeBiologyChlamydomonasReference genomeComputational biologyGenome sizeGenomicsEvolutionary biologyGeneticsGeneNanotechnologyTranscriptomeMaterials scienceMutantGene expressionPhotosynthetic Processes and MechanismsGenomics and Phylogenetic StudiesFish biology, ecology, and behavior