Pseudomonas guryensis sp. nov. and Pseudomonas ullengensis sp. nov., isolated from soil

Abstract

Two Gram-staining-negative, aerobic, rod-shaped bacteria designated strains SR9 T and UL070 T , were isolated from soil and subjected to taxonomic characterization. Strain SR9 T grew at 10–37 °C (optimum 30 °C), at pH 4.0–10.0 (optimum pH 8.0) and in the presence of 0–1 % NaCl (optimum 0 %), and UL070 T at 4–33 °C (optimum 30 °C), at pH 4.0–10.0 (optimum pH 7.0) and in the presence of 0–2 % NaCl (optimum 0 %), respectively. Strain UL070 T was motile with flagella. Analysis of 16S rRNA gene sequences indicated that the two strains fell into phylogenetic clusters belonging to the genus Pseudomonas . Both strains SR9 T and UL070 T were mostly related to Pseudomonas campi S1-A32-2 T with 99.70 and 99.01% sequence similarities, and the similarity between the two isolates was 98.90 %. The genome-based in silico analyses indicated that each of the strains SR9 T and UL070 T was clearly separated from other species of Pseudomonas , as the orthologous average nucleotide identity (OrthoANI) and the digital DNA–DNA hybridization (dDDH) values were no higher than 93.09 and 50.03% respectively with any related species, which were clearly below the cutoff for species distinction. The fatty acid profiles of the two strains mainly consisting of unsaturated components, the presence of ubiquinone 9 (Q-9) as the major respiratory quinone, and phosphatidylethanolamine (PE) and diphosphatidylglycerol (DPG) as the diagnostic polar lipids were consistent with their classification into Pseudomonas . The DNA G+C contents of strains SR9 T and UL070 T were 63.2 mol% and 63.6 mol% respectively. On the basis of both phenotypic and phylogenetic evidences, each of the isolated strains should be classified as a novel species, for which the names Pseudomonas guryensis sp. nov. (type strain=SR9 T =KCTC 82228 T =JCM 34509 T ) and Pseudomonas ullengensis sp. nov. (type strain=UL070 T =KCTC 82229 T =JCM 34510 T ) are proposed.

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