Structure-guided evolution of a ketoreductase for efficient and stereoselective bioreduction of bulky α-amino β-keto esters
Jiawei Tang, Liuqing Chen, Luwen Zhang, Guowei Ni, Jun Yu, Hongyi Wang, Fuli Zhang, Shuguang Yuan, Meiqing Feng, Shaoxin Chen
Abstract
Chiral vicinal amino alcohols were generated with excellent stereoselectivity and high conversion from bulky α-amino β-keto esters by an engineered ketoreductase called M30.
Topics & Concepts
StereoselectivityChemistryCombinatorial chemistryCatalysisAmino acidStereochemistryOrganic chemistryBiochemistryEnzyme Catalysis and ImmobilizationMicrobial Metabolic Engineering and BioproductionEnzyme Structure and Function