Rapid ultraviolet photoacoustic remote sensing microscopy using voice-coil stage scanning
Brendyn D. Cikaluk, Brendon S. Restall, Nathaniel J. M. Haven, Matthew T. Martell, Ewan A. McAlister, Roger J. Zemp
Abstract
There is an unmet need for fast virtual histology technologies that exhibit histological realism and can scan large sections of fresh tissue within intraoperative time-frames. Ultraviolet photoacoustic remote sensing microscopy (UV-PARS) is an emerging imaging modality capable of producing virtual histology images that show good concordance to conventional histology stains. However, a UV-PARS scanning system that can perform rapid intraoperative imaging over mm-scale fields-of-view at fine resolution (<500 nm) has yet to be demonstrated. In this work, we present a UV-PARS system which utilizes voice-coil stage scanning to demonstrate finely resolved images for 2×2 mm 2 areas at 500 nm sampling resolution in 1.33 minutes and coarsely resolved images for 4×4 mm 2 areas at 900 nm sampling resolution in 2.5 minutes. The results of this work demonstrate the speed and resolution capabilities of the UV-PARS voice-coil system and further develop the potential for UV-PARS microscopy to be employed in a clinical setting.