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Optimized expression of large fragment DNA polymerase I from <i>Geobacillus stearothermophilus</i> in <i>Escherichia coli</i> expression system

Eva Agustriana, Isa Nuryana, Fina Amreta Laksmi, Kartika Sari Dewi, Hans Wijaya, Nanik Rahmani, Danu Risqi Yudiargo, Astadewi Ismadara, Helbert, Moch Irfan Hadi, Awan Purnawan, Apridah Cameliawati Djohan

2022Preparative Biochemistry & Biotechnology15 citationsDOI

Abstract

polymerase was successfully purified showing 813.56 U/mg of polymerase activity.

Topics & Concepts

Escherichia coliGeobacillus stearothermophilusFragment (logic)BiologyPolymerase chain reactionDNAPolymeraseMolecular biologyGeneticsMicrobiologyChemistryBacteriaThermophileGeneComputer scienceProgramming languageBacteriophages and microbial interactionsBacterial Genetics and BiotechnologyRNA and protein synthesis mechanisms
Optimized expression of large fragment DNA polymerase I from <i>Geobacillus stearothermophilus</i> in <i>Escherichia coli</i> expression system | Litcius