A smartphone-assisted colorimetric aptasensor based on aptamer and gold nanoparticles for visual, fast and sensitive detection of ZEN in maize
Liyuan Zhang, Jiayu Chen, Li-Feng Lu, Runzhong Yu, Dongjie Zhang
Abstract
A simple, fast, low cost, sensitive, intuitive, visual, label-free, and smartphone-assisted aptamer sensor based on colorimetric assay for the measurement of zearalenone was constructed. The nucleic acid aptamer of zearalenone was used as the recognition element and gold nanoparticles were used as the indicator. Several factors that could influence sensitivity, including the concentration of aptamer and NaCl, and incubation time, and specificity, have been investigated. The results showed that under the optimal conditions, the signal had a good linear relationship when zearalenone concentration is 5–300 ng/mL. A linear regression equation is Y = 0.0003X + 0.5128 (R2 = 0.9989) and a limit of detection is 5 ng/mL. The specificity of the sensor was good. Zearalenone in maize samples were successfully measured. The recoveries of Zearalenone are 81.3 %-96.4 %. The whole process takes only 15 min to complete. The smartphone assisted colorimetric aptamer sensor can be used for the detection of zearalenone in maize.