Synthesis of<i>Axinyssa digitata</i>Extract Directed Hybrid Nanoflower and Investigation of Its Antimicrobial Activity
Barış Karslı, İbrahim Seyda Uras, Belma Konuklugil, Ayşe Demırbas
Abstract
First time in this study, the antibacterial effects of <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Axinyssa digitata</i> sponge extracts and <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Axinyssa digitata</i> -based cupper hybrid nanoflowers (Cu hNFs) were evaluated. Herein, hybrid nanoflowers (Cu hNFs) were produced by combining <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Axinyssa digitata</i> sponge extract with Cu <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2+</sup> ions in Phosphate-buffered saline (PBS) (at pH 7.4) at room temperature for three days using green synthesis method. The shape and size of hNFs were evaluated using scanning electron microscope (SEM) images. Energy dispersive X-ray spectroscopy (EDX) mapping was used to determine the presence of Cu metals and other components. X-ray diffraction (XRD) is a non-destructive analysis method that was used to determine of the crystallographic properties of materials and the phases they contain. Fourier-transform infrared spectroscopy (FT-IR) peaks were used to discuss the presence of functional groups that played a key role in the synthesis. The Cu-hNFs had antimicrobial activity against selected microorganisms. This research is expected to provide knowledge on hNFs synthesis and antimicrobial activity application investigations using <italic xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">Axinyssa digitata</i> rather than biomolecules obtained through costly and time-consuming methods.