Genetic Dissection of Antibiotic Adjuvant Activity
Jeannie Bailey, Larry A. Gallagher, William T. Barker, Veronica B. Hubble, Joe Gasper, Christian Melander, Colin Manoil
Abstract
One strategy to confront the antibiotic resistance crisis is through the development of adjuvant compounds that increase the efficacy of established drugs. A key step in the development of a natural product adjuvant as a drug is identifying the resistance process it undermines to enhance antibiotic activity. Previous procedures designed to accomplish this have relied on biochemical identification of cell components that bind adjuvant. Here, we present a complementary strategy based on identifying mutations that eliminate adjuvant activity.
Topics & Concepts
Acinetobacter baumanniiAntibioticsAdjuvantMicrobiologyMeropenemAntibiotic resistanceBacterial outer membraneChemistryBiologyBacteriaBiochemistryGeneImmunologyPseudomonas aeruginosaEscherichia coliGeneticsAntibiotic Resistance in BacteriaBacterial biofilms and quorum sensingBacterial Genetics and Biotechnology