The establishment of a homozygous SNTA1 knockout human embryonic stem cell line (WAe009-A-50) using the CRISPR/Cas9 system
Tao Dong, Siyao Zhang, Yun Chang, Rui Bai, Youxu Jiang, Shuhong Ma, Yanan Li, Hong Jiang, Wenjing Lu
Abstract
SNTA1 encodes α1-syntrophin, a scaffold protein, which is a component of the dystrophin-associated protein complex. Additionally, α1-syntrophin interacts with SCN5A and nNOS-PMCA4b complex in cardiomyocytes. SNTA1 is a susceptibility locus for arrhythmia and cardiomyopathy. We generated a homozygous SNTA1 knockout human embryonic stem cell (H9SNTA1KO) using the CRISPR/Cas9 system. H9SNTA1KO maintained pluripotency and a normal karyotype and differentiated into three germ layers in vivo.
Topics & Concepts
BiologyCRISPREmbryonic stem cellDystrophinGerm layerGermlineCell biologyStem cellGene knockinCas9Knockout mouseGeneticsMolecular biologyInduced pluripotent stem cellDuchenne muscular dystrophyGeneCRISPR and Genetic EngineeringRNA Research and SplicingPluripotent Stem Cells Research