Efficient opto- and chemogenetic control in a single molecule driven by FRET-modified bioluminescence
Andreas Björefeldt, Jeremy W. Murphy, Emmanuel L. Crespo, Gerard G. Lambert, Mansi Prakash, Ebenezer C Ikefuama, Nina Friedman, Tariq M. Brown, Diane Lipscombe, Christopher I. Moore, Ute Hochgeschwender, Nathan C. Shaner
Abstract
Significance: Bioluminescent optogenetics (BL-OG) offers a unique and powerful approach to manipulate neural activity both opto- and chemogenetically using a single actuator molecule (a LuMinOpsin, LMO). Aim: To further enhance the utility of BL-OG by improving the efficacy of chemogenetic (bioluminescence-driven) LMO activation. Approach: Channelrhodopsin 1 (VChR1). Results: , and efficient modulation of behavior. Conclusions: We report a robust new option for achieving multiple modes of control in a single actuator and a promising engineering strategy for continued improvement of BL-OG.