Endoplasmic reticulum membrane receptors of the GET pathway are conserved throughout eukaryotes
Lisa Yasmin Asseck, Dietmar Gerald Mehlhorn, Jhon Erick Rivera‐Monroy, Martiniano M. Ricardi, Holger Breuninger, Niklas Wallmeroth, Kenneth Wayne Berendzen, Minou Nowrousian, Shuping Xing, Blanche Schwappach, Martin Bayer, Christopher Grefen
Abstract
Significance The GET pathway is required for the insertion of tail-anchored (TA) membrane proteins in the endoplasmic reticulum (ER) of yeast and mammals. Some orthologous genes had also been identified in higher plants with the exception of one of the two ER membrane receptors required for membrane insertion. Get2/CAML is required for the pathway’s cytosolic chaperone to dock and release its TA protein cargo. Here we report the identification of the elusive plant GET pathway receptor through an interaction screen in Arabidopsis . The candidate allows detection of further Get2/CAML orthologs in higher plants, revealing conservation and function of structural features across kingdoms. Additionally, our results demonstrate that these features, rather than sequence conservation, determine functionality of the candidate within the pathway.