Ultrasensitive and ratiometric two-photon fluorescence imaging of Golgi polarity during drug-induced acute kidney injury
Hui Wang, Mingyan Dong, Hongtong Wang, Fang Huang, Ping Li, Wei Zhang, Wen Zhang, Bo Tang
Abstract
We synthesized an ultrasensitive probe TP-Golgi for the two-photon ratiometric fluorescence imaging of Golgi polarity. Probe TP-Golgi possesses a large Stokes shift, excellent sensitivity and good selectivity to quantitatively detect environmental polarity. By application of TP-Golgi, we found that the Golgi polarity increased obviously in the kidneys of mice with AKI.
Topics & Concepts
Golgi apparatusPolarity (international relations)FluorescenceChemistryFluorescence-lifetime imaging microscopyTwo-photon excitation microscopyBiophysicsStokes shiftBiologyBiochemistryOpticsCellPhysicsAcute Kidney Injury ResearchProtein Interaction Studies and Fluorescence AnalysisRetinal Development and Disorders