Engineering<i>Yarrowia lipolytica</i>for<i>de novo</i>production of tetraacetyl phytosphingosine
Changpyo Han, Min‐Jeong Jang, Min Ju Kim, Man-ho Han, Kyeong‐Ryoon Lee, Ji‐Sook Hahn, Jungoh Ahn
Abstract
AIMS: To genetically engineer the oleaginous yeast Yarrowia lipolytica for de novo production of tetraacetylphytosphingosine (TAPS), a precursor of phytosphingosine, and optimization of fermentation conditions for high yield. METHODS AND RESULTS: of TAPS production within 56 h of the fermentation. CONCLUSIONS: The introduction of codon-optimized Sli1p and Atf2p, deletion of LCB4 gene and sexual hybridization, accompanied by specific fermentation conditions, enhanced TAPS yield in Y. lipolytica. SIGNIFICANCE AND IMPACT OF THE STUDY: Our results highlight Y. lipolytica as a promising candidate for the industrial production of TAPS, an important component of cosmetic formulations.