Impact of broad-spectrum antibiotics on the gut–microbiota–spleen–brain axis
Xiayun Wan, Akifumi Eguchi, Akemi Sakamoto, Yūkō Fujita, Yong Yang, Youge Qu, Masahiko Hatano, Chisato Mori, Kenji Hashimoto
Abstract
T cells. Furthermore, ABX treatment resulted in the depletion of a large portion of the gut microbiota. Untargeted metabolomics analysis showed that ABX treatment caused alterations in the levels of certain compounds in the plasma, spleen, and brain. Moreover, ABX treatment decreased the expression of microglia marker Iba1 in the cerebral cortex. Interestingly, correlations were found between the abundance of different microbiome components and metabolites in various tissues, as well as splenic cell populations and spleen weight. These findings suggest that ABX-induced microbiome depletion and altered metabolite levels may affect spleen and brain function through the gut-microbiota-spleen-brain axis.