Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
Kei Takahashi, Shimpei I. Kubota, Shogo Ehata, Hiroki R. Ueda, Kohei Miyazono
Abstract
Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry. For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).
Topics & Concepts
ClearingProtocol (science)Computer scienceCancerBiomedical engineeringArtificial intelligencePathologyMedicineInternal medicineFinanceAlternative medicineEconomicsCell Image Analysis TechniquesCancer Cells and MetastasisAdvanced Fluorescence Microscopy Techniques