Sanger Tree of Life Wet Laboratory Protocol Collection v1
Amy Denton, Halyna Yatsenko, Jessie Jay, Karen E Houliston, Caroline Howard
Abstract
This is the collection of Sanger Tree of Life Wet Laboratory protocols used for the generation of reference level genome assemblies. This collection currently contains protocols which cover all stages of the wet laboratory processes performed by the Sanger Tree of Life; these processes are sample preparation, sample homogenisation, HMW DNA extraction, HMW DNA fragmentation, fragmented DNA clean-up and RNA extraction. This collection will be updated as protocols within the Tree of Life Core Laboratory continue to be developed and refined.
Topics & Concepts
Protocol (science)Tree of life (biology)Tree (set theory)Sanger sequencingComputational biologyComputer scienceBiologyDNA sequencingDNAMedicineMathematicsGeneticsPathologyGeneAlternative medicinePhylogeneticsMathematical analysisMolecular Biology Techniques and Applications