Fluorescence lifetime predicts performance of voltage sensitive fluorophores in cardiomyocytes and neurons
Steven Boggess, Julia R. Lazzari-Dean, Benjamin K. Raliski, Dong Min Mun, Amy Y. Li, Joshua L. Turnbull, Evan W. Miller
Abstract
per mV), coupled with traditional fluorescence intensity-based metrics like ΔF/F and signal-to-noise ratio (SNR), provides a powerful method to both predict and understand indicator performance in cellular systems.
Topics & Concepts
FluorescenceElectrodeFluorescence-lifetime imaging microscopyVoltageComplement (music)Materials scienceOptoelectronicsBiophysicsChemistryPhysicsOpticsElectrical engineeringBiologyEngineeringBiochemistryPhenotypeComplementationGenePhysical chemistryNeuroscience and Neural EngineeringElectrochemical Analysis and ApplicationsPhotoreceptor and optogenetics research