Overview of <i>in vitro</i> digestion methods to evaluate bioaccessibility of lipophilic compounds in foods
Nerea Faubel, Antonio Cilla, Amparo Alegrı́a, Reyes Barberá, Guadalupe García‐Llatas
Abstract
In vitro digestion models for assessing the bioaccessibility of lipophilic compounds from foodstuffs can either be static or dynamic. Carotenoids are the most widely studied compounds, while there is hardly any information on sterols and fat-soluble vitamins available, nor on the use of dynamic methods. There is a large variability in the parameters of static methods, which makes data comparison difficult. To harmonize digestion conditions and compare bioaccessibility, INFOGEST model has been established. This review assesses the most relevant digestion methods (non-INFOGEST, INFOGEST and dynamic) applied to carotenoids, sterols, and fat-soluble vitamins in food matrices in the last ten years.