A new electrochemical DNA biosensor for determination of anti-cancer drug chlorambucil based on a polypyrrole/flower-like platinum/NiCo<sub>2</sub>O<sub>4</sub>/pencil graphite electrode
Hadi Mahmoudi‐Moghaddam, Zahra Garkani‐Nejad
Abstract
/PGE was able to detect the different concentrations of CHB in the range between 0.018 and 200 μM, with a detection limit of (LOD) of 4.0 nM. The new biosensor was also exploited for CHB determination in real samples (serum, urine and drug), the results of which revealed excellent recoveries (97.5% to 103.8%). Furthermore, the interaction between ds-DNA and CHB was studied using electrochemistry, spectrophotometry and docking whose outputs confirmed their effective interaction.
Topics & Concepts
PlatinumPolypyrroleChlorambucilBiosensorElectrochemistryPencil (optics)GraphiteElectrodeDrugMaterials scienceDNAChemistryNanotechnologyOrganic chemistryPharmacologyBiochemistryPhysical chemistryMedicineChemotherapyOpticsInternal medicinePhysicsCatalysisCyclophosphamideAdvanced biosensing and bioanalysis techniquesMolecular Junctions and NanostructuresElectrochemical sensors and biosensors