NaGdF<sub>4</sub>:Nd@NaGdF<sub>4</sub> Core-Shell Down-Conversion Nanoparticles as NIR-II Fluorescent Probes for Targeted Imaging of Bacteria
Leilei Sun, Sisi Shi, Hongchao Geng, Yao Huang, Yan Qiao, Jie Song, Lan Yang, Craig A. Grimes, Xinxin Feng, Qingyun Cai
Abstract
Bacterial infections are a serious ongoing threat to human health. As such, within the context of modern technology, there is an urgent need to develop fluorescent probes suitable for diagnosing bacterial infections in vivo. Herein, we synthesize NaGdF4:Nd@NaGdF4 core-shell down-conversion nanoparticles (DCNPs) demonstrating excellent emission intensity in the second near-infrared (NIR-II) window for use as fluorescent probes to enable high-quality imaging of pathogenic infections. The fluorescent probe is covalently linked with vancomycin (Van), which specifically binds to Gram-positive bacteria such as S. aureus. Both in vitro detection and in vivo imaging results show that the Van-modified NIR-II fluorescent probe can distinguish Gram-positive from Gram-negative bacteria with a high degree of selectivity. Biocompatibility is verified by MTT assay and histological analyses.