Synthesis of <scp>l</scp>-aspartic acid-based bimetallic hybrid nanoflowers to immobilize snailase for the production of rare ginsenoside compound K
Duoduo Tian, Zhansheng Wu, Xiaochen Liu, Zhuo Tu, Runze Li, Daidi Fan, Yiran Lan
Abstract
. The more abundant mesopores allowed the enzyme to maintain a good conformation and the enzyme activity was 79.8% of that of the free Sna. In addition, the total conversion rate of Asp@ZIF-ZnCo-Sna to ginsenoside Rb1 was as high as 88.35%, whereas that of ZIF-ZnCo-Sna was 79.12%. Moreover, after 6 cycles, the catalytic conversion of ZIF-ZnCo-Sna and Asp@ZIF-ZnCo-Sna and the crystalline shape remained the same, indicating that both composites have good stability and catalytic properties. This new approach of improving the MOF morphology and enzymatic activity by a one-step addition of small biological molecules provides a simple, rapid, and effective strategy for biocatalysis. It also provides a certain reference value for the immobilized Sna to produce rare ginsenoside CK.