Microsecond Folding of preQ <sub>1</sub> Riboswitch and Its Biological Significance Revealed by Two-Dimensional Fluorescence Lifetime Correlation Spectroscopy
Bidyut Sarkar, Kunihiko Ishii, Tahei Tahara
Abstract
substantially stabilizes the fF form that is generated from the pF form with a long time constant (>10 ms). Combining these results with the results of a former smFRET study on the slower time scale, we obtain an overall picture of the folding/unfolding dynamics of the aptamer domain as well as its energy landscape. On the basis of the picture obtained, we discuss the significance of the microsecond folding/unfolding of the aptamer domain for biological function of the riboswitch and propose the molecular mechanism of the gene expression controlled by the structural dynamics of the aptamer domain.
Topics & Concepts
ChemistryAptamerRiboswitchPseudoknotFolding (DSP implementation)MicrosecondBiophysicsMolecular dynamicsConformational changeCrystallographyOligonucleotideStereochemistryRNABiochemistryDNAComputational chemistryBiologyGenePhysicsGeneticsNon-coding RNAEngineeringAstronomyElectrical engineeringRNA and protein synthesis mechanismsBacterial Genetics and BiotechnologyNanopore and Nanochannel Transport Studies