Postmodification via Thiol-Click Chemistry Yields Hydrophilic Trityl-Nitroxide Biradicals for Biomolecular High-Field Dynamic Nuclear Polarization
Weixiang Zhai, Alessandra Lucini Paioni, Xinyi Cai, Siddarth Narasimhan, João Medeiros‐Silva, Wenxiao Zhang, Antal Rockenbauer, Markus Weingarth, Yuguang Song, Marc Baldus, Yangping Liu
Abstract
N-proline in a glycerol/water matrix are inversely correlated with their hydrophobicity. Importantly, applications of hydrophilic NATriPol-5 and NATriPol-3 to biomolecules including a globular soluble protein and a membrane targeting peptide reveal significantly improved performance compared to TEMTriPol-1 and AMUPol. Our work provides an efficient approach for one-step synthesis of new polarizing agents with tunable physicochemical properties, thus expediting optimization of new biradicals for biomolecular applications at ultrahigh magnetic fields.
Topics & Concepts
Click chemistryNitroxide mediated radical polymerizationBiomoleculeChemistryCombinatorial chemistryThiolMoleculeOrganic chemistryCopolymerPolymerBiochemistryRadical polymerizationAdvanced NMR Techniques and ApplicationsSolid-state spectroscopy and crystallographyElectron Spin Resonance Studies