Rapid Diagnosis of Mycobacterium marinum Infection by Next-Generation Sequencing: A Case Report
Fanfan Xing, Simon K. F. Lo, Yuanchao Ma, Jonathan Daniel Ip, Wan-Mui Chan, Meixun Zhou, Miaozi Gong, Susanna K. P. Lau, Patrick C. Y. Woo
Abstract
infection diagnosed by next-generation sequencing (NGS). It took <2 days to make a microbiological diagnosis using the Oxford Nanopore Technologies' MinION device, compared to 20 days for the mycobacterium to be isolated from the tissue biopsy. NGS is particularly useful for culture-negative and slow-growing microorganism infections, such as mycobacterial, fungal and partially treated pyogenic bacterial infections. Due to its low equipment cost, short turn-around-time and portable size, the Oxford Nanopore Technologies' MinION device is a useful platform for NGS in routine clinical microbiology laboratories.