RPAcan3990: an Ultrasensitive Recombinase Polymerase Assay To Detect <i>Angiostrongylus cantonensis</i> DNA
William J. Sears, Yvonne Qvarnström, Thomas B. Nutman
Abstract
sp.) were negative in the RPAcan3990 assay. The optimal incubation temperature range for the reaction was between 35°C and 40°C. The assay successfully detected 1 fg/μl of A. cantonensis genomic DNA after incubation at human body temperature (in a shirt pocket). In conclusion, these data suggest RPAcan3990 is potentially a point-of-contact molecular assay capable of sensitively detecting A. cantonensis by producing visually interpretable results with minimal instrumentation.
Topics & Concepts
Angiostrongylus cantonensisBiologygenomic DNAAngiostrongyliasisRecombinase Polymerase AmplificationPolymerase chain reactionMolecular biologyVirologyMicrobiologyDNAImmunologyGeneticsHelminthsGeneMollusks and Parasites StudiesParasite Biology and Host InteractionsParasitic Infections and Diagnostics