CRAGE-Duet Facilitates Modular Assembly of Biological Systems for Studying Plant–Microbe Interactions
Bing Wang, Zhiying Zhao, Lauren K. Jabusch, Dawn Chiniquy, Koyo Ono, Jonathan M. Conway, Zheyun Zhang, Gaoyan Wang, David S. Robinson, Jan‐Fang Cheng, Jeffery L. Dangl, Trent R. Northen, Yasuo Yoshikuni
Abstract
WCS417r that expressed various fluorescence marker genes. We visualized these strains simultaneously under a confocal microscope, demonstrating the usefulness of CRAGE-Duet for creating biological systems to study plant-microbe interactions.
Topics & Concepts
Modular designBiologyComputational biologyRecombinaseCas9Biochemical engineeringBiotechnologyComputer scienceCRISPRGeneGeneticsEngineeringRecombinationOperating systemPlant-Microbe Interactions and ImmunityCRISPR and Genetic EngineeringPlant tissue culture and regeneration