Hybrid double-spiral microfluidic chip for RBC-lysis-free enrichment of rare cells from whole blood
Kentaro Shirai, Guofeng Guan, Tan Meihui, Peng Xiaoling, Yuma Oka, Yusuke Takahashi, Ali Asgar S. Bhagat, Masatoshi Yanagida, Shigeki Iwanaga, Nobuaki Matsubara, Toru Mukohara, Tomokazu Yoshida
Abstract
Hybrid double-spiral chip was developed to enrich circulating tumor cells (CTCs) without red blood cell lysis for liquid biopsy. Combined with the imaging flow cytometry, CTCs detection from cancer patient blood was demonstrated.
Topics & Concepts
Circulating tumor cellLysisWhole bloodLiquid biopsyCentrifugationFlow cytometryCancer cellCancerLysis bufferRed blood cellBiomedical engineeringChemistryBiologyChromatographyMedicineMolecular biologyImmunologyMetastasisInternal medicineBiochemistryMicrofluidic and Bio-sensing TechnologiesNanopore and Nanochannel Transport StudiesCancer Cells and Metastasis