Phenotypic Detection and Differentiation of Carbapenemase Classes Including OXA-48-Like Enzymes in <i>Enterobacterales</i> and <i>Pseudomonas aeruginosa</i> by a Highly Specialized Micronaut-S Microdilution Assay
Niels Pfennigwerth, Sören Gatermann, Barbara Körber-Irrgang, Robert Hönings
Abstract
The objective of this study was to evaluate the Micronaut-S carbapenemase detection microtiter plate assay for the detection of carbapenemases and Ambler class determination. The Micronaut-S carbapenemase detection microtiter plate was tested using a challenging collection of 154 carbapenemase-producing and 150 carbapenemase-negative clinical strains of Enterobacterales and Pseudomonas aeruginosa . The Micronaut-S carbapenemase detection assay was able to detect 148/154 carbapenemase producers correctly, whereas 5/150 non-carbapenemase-producing isolates tested as false positive.
Topics & Concepts
Pseudomonas aeruginosaBroth microdilutionMicrobiologyBiologyMicrotiter platePhenotypic screeningEnterobacteriaceaeBacteriaPhenotypeMolecular biologyEscherichia coliAntimicrobialMinimum inhibitory concentrationGeneGeneticsAntibiotic Resistance in BacteriaBacterial Identification and Susceptibility TestingEnterobacteriaceae and Cronobacter Research