Global fungal-host interactome mapping identifies host targets of candidalysin
Tianyi Zhang, Yaoqi Chen, Jing-Cong Tan, Jin‐An Zhou, Wan-Ning Chen, Tong Jiang, Jinyin Zha, Xiang-Kang Zeng, Bowen Li, Luqi Wei, Yun Zou, Luyao Zhang, Yue-Mei Hong, Xiuli Wang, Run‐Ze Zhu, Wanxing Xu, Jing Xi, Qinqin Wang, Lei Pan, Jian Zhang, Yang Luan, Ruixin Zhu, Hui Wang, Changbin Chen, Ning‐Ning Liu
Abstract
Candidalysin, a cytolytic peptide toxin secreted by the human fungal pathogen Candida albicans, is critical for fungal pathogenesis. Yet, its intracellular targets have not been extensively mapped. Here, we performed a high-throughput enhanced yeast two-hybrid (HT-eY2H) screen to map the interactome of all eight Ece1 peptides with their direct human protein targets and identified a list of potential interacting proteins, some of which were shared between the peptides. CCNH, a regulatory subunit of the CDK-activating kinase (CAK) complex involved in DNA damage repair, was identified as one of the host targets of candidalysin. Mechanistic studies revealed that candidalysin triggers a significantly increased double-strand DNA breaks (DSBs), as evidenced by the formation of γ-H2AX foci and colocalization of CCNH and γ-H2AX. Importantly, candidalysin binds directly to CCNH to activate CAK to inhibit DNA damage repair pathway. Loss of CCNH alleviates DSBs formation under candidalysin treatment. Depletion of candidalysin-encoding gene fails to induce DSBs and stimulates CCNH upregulation in a murine model of oropharyngeal candidiasis. Collectively, our study reveals that a secreted fungal toxin acts to hijack the canonical DNA damage repair pathway by targeting CCNH and to promote fungal infection.