A polyamino acid-based phosphatidyl polymer library for <i>in vivo</i> mRNA delivery with spleen targeting ability
Hanqin Zhao, Sheng Ma, Yibo Qi, Yuxi Gao, Yuyan Zhang, Minhui Li, Jie Chen, Wantong Song, Xuesi Chen
Abstract
studies proved that PLG-PPs/mRNA polyplexes exhibited a significant increase in mRNA expression, peaking 14 716 times compared to their non-phosphatidyl parent polymer. Impressively, the subset PA8-PL3 not only facilitated efficient mRNA transfection but also selectively delivered mRNA to the spleen instead of the liver (resulting in 69.73% protein expression in the spleen) once intravenously administered. This type of phosphatidyl PLG polymer library provides a novel approach to the construction of mRNA delivery systems especially for spleen-targeted mRNA therapeutic delivery.
Topics & Concepts
In vivoSpleenMessenger RNACell biologyChemistryBiochemistryMedicineBiologyImmunologyBiotechnologyGeneRNA Interference and Gene DeliveryAdvanced biosensing and bioanalysis techniquesPhagocytosis and Immune Regulation