Theophylline-induced synergic activation of guide RNA to control CRISPR/Cas9 function
Yan Liu, Yang Wang, Jiao Lin, Liang Xu
Abstract
Ligand-induced activation of CRISPR/Cas9 function is achieved based on a synergic approach through the integration of the theophylline aptamer into protein-unrecognized regions of guide RNA. This design of allosteric regulation opens a new window towards the broad involvement of RNA aptamers for conditional control of CRISPR/Cas9 function.
Topics & Concepts
CRISPRRNATheophyllineChemistryFunction (biology)Computational biologyBiologyGeneticsBiochemistryGenePharmacologyCRISPR and Genetic EngineeringAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene Delivery