Development and evaluation of polyclonal antibodies based antigen capture ELISA for detection of porcine rotavirus
Atta Muhammad Memon, Fangzhou Chen, Sher Bahadar Khan, Xiaozhen Guo, Rajwali Khan, Farhan Anwar Khan, Yinxing Zhu, Qigai He
Abstract
) between these two techniques. Furthermore, AC-ELISA could not detect any cross-reactivity with porcine epidemic diarrhea virus, transmissible gastro-enteritis virus, pseudo rabies virus and porcine circovirus-2. This in-house AC-ELISA efficiently detected PoRVA from clinical samples, which suggests that this technique can be used for large-scale surveillance and timely detection of rotavirus infection in the porcine farms.
Topics & Concepts
Polyclonal antibodiesVirologyPorcine epidemic diarrhea virusRotavirusBiologyAntibodyPorcine circovirusAntigenVirusTaqManMolecular biologyDetection limitReal-time polymerase chain reactionChemistryImmunologyGeneChromatographyBiochemistryViral gastroenteritis research and epidemiologyAnimal Virus Infections StudiesViral Infections and Immunology Research