Influenza A virus causes maternal and fetal pathology via innate and adaptive vascular inflammation in mice
Stella Liong, Osezua Oseghale, Eunice E. To, Kurt Brassington, Jonathan R. Erlich, Raymond Luong, Felicia Liong, Robert D. Brooks, Cara Martin, Sharon O’Toole, Antony Vinh, Luke O'neill, Steven Bozinovski, Ross Vlahos, Paris C. Papagianis, John O’Leary, Doug A. Brooks, Stavros Selemidis
Abstract
monocytes, plus neutrophils and T cells. This vascular storm was associated with elevated levels of the adhesion molecules ICAM and VCAM and the pattern-recognition receptors TLR7 and TLR9 in the vascular wall, resulting in profound vascular dysfunction. The sequalae of this IAV-driven vascular storm included placental growth retardation and intrauterine growth restriction, evidence of placental and fetal brain hypoxia, and increased circulating cell free fetal DNA and soluble Flt1. In contrast, IAV infection in nonpregnant mice caused no obvious alterations in endothelial function or vascular inflammation. Therefore, IAV infection during pregnancy drives a significant systemic vascular alteration in pregnant dams, which likely suppresses critical blood flow to the placenta and fetus. This study in mice provides a fundamental mechanistic insight and a paradigm into how an immune response to a respiratory virus, such as IAV, is likely to specifically drive maternal and fetal pathologies during pregnancy.