Optimizing tobacco (Nicotiana tabacum L.) carbon metabolism regulation via topping: A study based on multi-omics
Nuo Shi, Meng Jia, Xuanquan Zhu, Qi Luo, Yuxiang Bai, Na Wang, Yu Du, Dingchun Zi, Limei Zhang, Tikun Zhang, Ge Wang, Peng Zhou
Abstract
Topping eliminates the apical dominance of tobacco, alters the structure of tobacco leaves, and regulates the chemical composition of tobacco leaves. This study investigated how different topping periods affect photosynthetic carbon metabolism and related regulatory mechanisms in tobacco leaves through integrated physiological, proteomic, and metabolomic analyses. The results indicated that topping could effectively promote the broadening of leaves and the increase of leaf area, and significantly enhance the photosynthetic rate of leaves. Furthermore, topping promotes the Calvin cycle and sucrose metabolism in photosynthetic organisms, increasing starch content and reducing sucrose content. Overall, topping promotes photosynthetic carbon metabolism. These results not only contribute a theoretical basis for the response mechanism and physiological changes in tobacco as the target products under different topping periods but also providing theoretical support for optimizing tobacco planting practices.