The critical role of Asp206 stabilizing residues on the catalytic mechanism of the <i>Ideonella sakaiensis</i> PETase
Rita P. Magalhães, Henrique S. Fernandes, Sérgio F. Sousa
Abstract
We described the catalytic mechanism of Is PETase, a polyethylene-terephthalate degrading enzyme. The reaction was found to progress in four steps, divided in two events: formation of the first transition intermediate and hydrolysis of the adduct.
Topics & Concepts
CatalysisMechanism (biology)AdductChemistryHydrolysisReaction mechanismCombinatorial chemistryOrganic chemistryPhysicsQuantum mechanicsGraphene and Nanomaterials ApplicationsBiotin and Related Studies