Toward Kilogram-Scale Peroxygenase-Catalyzed Oxyfunctionalization of Cyclohexane
Thomas Hilberath, Remco van Oosten, Juliet Victoria, Hugo Brasselet, Miguel Alcalde, John M. Woodley, Frank Hollmann
Abstract
High Resolution Image Download MS PowerPoint Slide Mol-scale oxyfunctionalization of cyclohexane to cyclohexanol/cyclohexanone (KA-oil) using an unspecific peroxygenase is reported. Using Aae UPO from Agrocybe aegerita and simple H 2 O 2 as an oxidant, cyclohexanol concentrations of more than 300 mM (>60% yield) at attractive productivities (157 mM h –1, approx. 15 g L –1 h –1 ) were achieved. Current limitations of the proposed biooxidation system have been identified paving the way for future improvements and implementation.
Topics & Concepts
CyclohexaneKilogramCatalysisChemistryOrganic chemistryBody weightBiologyEndocrinologyEnzyme Catalysis and ImmobilizationMicrobial bioremediation and biosurfactantsElectrochemical sensors and biosensors