Dual-Luciferase-Based Fast and Sensitive Detection of Malaria Hypnozoites for the Discovery of Antirelapse Compounds
Annemarie Voorberg-van der Wel, Anne‐Marie Zeeman, Ivonne G. Nieuwenhuis, Nicole M. van der Werff, Els J. Klooster, Onny Klop, Lars Vermaat, Clemens H. M. Kocken
Abstract
promoter. The transgenic parasite line showed similar fitness and drug sensitivity profiles of selected compounds to wild type. We demonstrate robust bioluminescence-based detection of hypnozoites in 96-well and 384-well plate formats, setting the stage for implementation in large scale drug screens.
Topics & Concepts
LuciferaseMalariaParasite hostingTransgeneDrug discoveryVirologyBiopharmaceuticalBiologyChemistryImmunologyBiotechnologyBiochemistryGeneTransfectionComputer scienceWorld Wide WebMalaria Research and ControlMosquito-borne diseases and controlComputational Drug Discovery Methods