Rapid Nanopore Whole-Genome Sequencing for Anthrax Emergency Preparedness
Heather P. McLaughlin, Julia V. Bugrysheva, Andrew B. Conley, Christopher A. Gulvik, Blake Cherney, Cari B. Kolton, Chung K. Marston, Elke Saile, Erin Swaney, David Lonsway, Amy S. Gargis, Thiphasone Kongphet-Tran, Christine Lascols, Pierre Michel, Julie Villanueva, Alex R. Hoffmaster, Jay E. Gee, David Sue
Abstract
Human anthrax cases necessitate rapid response. We completed Bacillus anthracis nanopore whole-genome sequencing in our high-containment laboratory from a human anthrax isolate hours after receipt. The de novo assembled genome showed no evidence of known antimicrobial resistance genes or introduced plasmid(s). Same-day genomic characterization enhances public health emergency response.
Topics & Concepts
Bacillus anthracisNanopore sequencingGenomeWhole genome sequencingBiologyAnthrax vaccinesShotgun sequencingMicrobiologyComputational biologyGeneticsPlasmidGeneBacteriaDNA vaccinationBacillus and Francisella bacterial researchBacteriophages and microbial interactionsBacterial Genetics and Biotechnology