Lysosome-Targeting Fluorescence Sensor for Sequential Detection and Imaging of Cu<sup>2+</sup> and Homocysteine in Living Cells
Lihua Liu, Hongfei Duan, Haohui Wang, Jieru Miao, Zhihui Wu, Chenxi Li, Yan Lu
Abstract
and Hcy in HeLa cells. More importantly, Hcy concentration was linearly related to the fluorescence intensity of PTNPy in living cells, demonstrating huge potential for real-time monitoring the fluctuation of Hcy levels in living cells.
Topics & Concepts
FluorescenceHeLaDetection limitChemistryQuenching (fluorescence)Fluorescence-lifetime imaging microscopyCytotoxicityConjugated systemHomocysteineSelectivityCysteineGlutathionePhotochemistryBiophysicsBiochemistryChromatographyCellPolymerBiologyOrganic chemistryIn vitroOpticsCatalysisEnzymePhysicsSulfur Compounds in BiologyMolecular Sensors and Ion DetectionFolate and B Vitamins Research