Unraveling the relationships between midge abundance and incidence, microbial communities, and soil and water properties in a protected natural tallgrass prairie
Saraswoti Neupane, Travis Davis, Cassandra Olds, Dana Nayduch, Bethany L. McGregor
Abstract
BACKGROUND: Biting midges (Culicoides spp.) are small blood-feeding flies (Diptera: Ceratopogonidae) that transmit numerous pathogens that impact animal and human health. The larvae of several Culicoides spp., including vectors, are often found in organically enriched, moist soil habitats. However, the influence of biotic (e.g., cohabiting fauna, potential prey taxa) and abiotic factors (e.g., soil or water properties, time) on abundance and incidence of larval Culicoides in natural habitats is not well understood. This study evaluated the relationships between bacterial and protistan communities, soil and water physicochemical properties, and the abundance and incidence of Culicoides species in larval habitats at the Konza Prairie Biological Station in Kansas. METHODS: Soil and water samples were collected monthly from March 2021 to February 2022 from four midge larval habitat sites, including three grazed (low-production cattle-grazed (LPCG), high-production cattle-grazed (HPCG), and bison-grazed sites) and one formally ungrazed (i.e., no managed large mammals) site. Midge incidence and abundance were evaluated using emergence assays, which assessed the number of adults emerging from collected soil samples, and bacterial and protistan communities in these samples were characterized through amplicon sequencing of the 16S and 18S rRNA genes. Physicochemical properties of water and soil were also analyzed. RESULTS: Irrespective of site, the highest midge abundance was reported in warmer months between March and September, except June. Moreover, the greatest midge abundance, incidence, and prevalence occurred at the HPCG and bison-grazed sites, which had a persistent water source. Specific lineages of bacterial and protistan communities, soil texture, organic matter, and total dissolved solids in water samples were directly associated with the abundance of Culicoides spp. that emerged from soil samples. CONCLUSIONS: Both biotic (bacterial and protistan communities, presence of host animals), and abiotic (soil and water properties, season) factors affected the abundance and incidence of Culicoides spp. in natural habitats. The results presented in this study expand our understanding of the ecological and environmental factors influencing larval ecology of biting midges in natural developmental substrates. These insights have important implications for identifying potential developmental sites, which can be used for targeted management of Culicoides.