CRISPR-empowered hybridization chain reaction amplification for an attomolar electrochemical sensor
Ling Wan, Jianfeng Ma, Jiasheng Yi, Yan Dong, Renjie Niu, Yan Su, Qian Li, Dan Zhu, Jie Chao, Shao Su, Chunhai Fan, Lianhui Wang, Ying Wan
Abstract
Rapid pathogen screening holds the key against certain viral infections, especially in an overwhelming pandemic. Herein, a CRISPR-empowered electrochemical biosensor was designed for the ultrasensitive detection of the avian influenza A (H7N9) virus gene sequence. Combining the CRISPR/Cas system, a signal-amplification strategy and a high-conductivity sensing substrate, the developed biosensor showed an ultrawide dynamic range, an ultralow detection limit, and excellent selectivity for H7N9 detection, providing a potential sensing platform for the simple, fast, sensitive, and on-site detection of infectious diseases.